human fetal lung fibroblast cell line imr90  (ATCC)

Journal: iScience

Article Title: Human SKI component SKIV2L regulates telomeric DNA-RNA hybrids and prevents telomere fragility

doi: 10.1016/j.isci.2024.111096

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Article Snippet: Stable clone of adult female human cervical adenocarcinoma HeLa cell line with long telomeres (HeLa1.3 cells); adult male human fibrosarcoma HT1080 cell line overexpressing telomase (HT1080 super-telomerase cells, HT1080-ST) (kindly provided by T. de Lange and J. Lingner, respectively); adult female human osteosarcoma U2OS cell line (ATCC); human embryonic kidney HEK293FT cell line (ATCC); and human female fetal lung fibroblast IMR90 cell line (Coriel Institute) were cultured in DMEM medium supplemented with 10% (v/v) fetal bovine serum (FBS, Sigma-Aldrich, F2442) and maintained at 37°C in 5% (v/v) CO 2 .

Techniques: Virus, Recombinant, Protease Inhibitor, Reverse Transcription, Blocking Assay, Mass Spectrometry, SYBR Green Assay, Flow Cytometry, Imaging, Mutagenesis, Cell Cycle Assay, shRNA, Software

Journal: Mutation research

Article Title: The ATM kinase signaling induced by the low-energy ?-particles emitted by 33 P is essential for the suppression of chromosome aberrations and is greater than that induced by the energetic ?-particles emitted by 32 P

doi: 10.1016/j.mrfmmm.2011.01.005

Figure Lengend Snippet: The β-particles emitted by 32P induce pan-nuclear γH2AX while the β-particles emitted by 33P and γ-rays induce foci. IMR90 fibroblasts were exposed to the β-particles emitted by 32Por 33Por γ-rays and analyzed at 1 h following the initial exposure. (A) Representative images are presented for each condition. (B) The numbers of γH2AX foci were greatest in cells exposed to 2 Gy γ-rays. High numbers of γH2AX foci were seen in cells exposed to ~0.36 Gy β-particles emitted by 33P. These experiments were performed two times.

Article Snippet: Cultivation, metabolic labelling and irradiation The normal diploid fetal human lung fibroblast cell line IMR90 (ATCC, Manassas, VA) was cultured in DMEM (Lonza, Basel, Switzerland) supplemented with 10% FBS (Atlanta Biologicals, Lawrenceville, GA).

Techniques:

Journal: Mutation research

Article Title: The ATM kinase signaling induced by the low-energy ?-particles emitted by 33 P is essential for the suppression of chromosome aberrations and is greater than that induced by the energetic ?-particles emitted by 32 P

doi: 10.1016/j.mrfmmm.2011.01.005

Figure Lengend Snippet: The β-particles emitted by 33P induce greater numbers of 53BP1 foci than the β-particles emitted by 32P. IMR90 fibroblasts were exposed to the β-particles emitted by 32Por 33Por γ-rays and analyzed at 1 h following the initial exposure. (A) Representative images are presented for each condition. (B) >10 53BP1 foci were seen in approximately 100% of cells exposed to either the β-particles emitted by 33Por γ-rays. Approximately 90% of cells exposed to the β-particles emitted by 32P contained less than 10 53BP1. (C) The numbers of γH2AX foci were greatest in cells exposed to 2 Gy γ-rays. High numbers of 53BP1 foci were seen in cells exposed to ~0.36 Gy β-particles emitted by 33P. These experiments were performed three times.

Article Snippet: Cultivation, metabolic labelling and irradiation The normal diploid fetal human lung fibroblast cell line IMR90 (ATCC, Manassas, VA) was cultured in DMEM (Lonza, Basel, Switzerland) supplemented with 10% FBS (Atlanta Biologicals, Lawrenceville, GA).

Techniques:

Journal: Mutation research

Article Title: The ATM kinase signaling induced by the low-energy ?-particles emitted by 33 P is essential for the suppression of chromosome aberrations and is greater than that induced by the energetic ?-particles emitted by 32 P

doi: 10.1016/j.mrfmmm.2011.01.005

Figure Lengend Snippet: DNA damage signaling is induced in IMR90 cells exposed to the β-particles emitted by 33Por 32P. ATM kinase-dependent signaling is identified by concurrent treatment with the selective ATM kinase inhibitor 10 μM KU55933. Cells were exposed to 1 mCi/2 ml 33P- or 32P-orthophoshate, harvested at either 30 min or 1 h and cytoplamic, soluble nuclear and micrococcal nuclease-digested chromatin fractions were prepared. Protein extracts were resolved and immunoblotted for (A) p53, (B) ATM and (C) CHK2 using both phosphospecific and generic antisera.

Article Snippet: Cultivation, metabolic labelling and irradiation The normal diploid fetal human lung fibroblast cell line IMR90 (ATCC, Manassas, VA) was cultured in DMEM (Lonza, Basel, Switzerland) supplemented with 10% FBS (Atlanta Biologicals, Lawrenceville, GA).

Techniques:

Journal: Mutation research

Article Title: The ATM kinase signaling induced by the low-energy ?-particles emitted by 33 P is essential for the suppression of chromosome aberrations and is greater than that induced by the energetic ?-particles emitted by 32 P

doi: 10.1016/j.mrfmmm.2011.01.005

Figure Lengend Snippet: Chromosome aberrations induced by 2 Gy γ-IR or ~0.36Gy β-particles in IMR90 fibroblasts when ATM was inhibited for 1 h.

Article Snippet: Cultivation, metabolic labelling and irradiation The normal diploid fetal human lung fibroblast cell line IMR90 (ATCC, Manassas, VA) was cultured in DMEM (Lonza, Basel, Switzerland) supplemented with 10% FBS (Atlanta Biologicals, Lawrenceville, GA).

Techniques: